No. 24

Jellyfish Sting

MARCH  2000

Significant Papers Published

hemolysin and phospholipase gel filtration eluant curves of Ca venom were similar. Venomous AaRS actively stung lips and contained more potent mouse lethal, demonecrotic, vasopermeability plus hemolytic factors than AaCB. Cross reactivity of convalescent human serum obtained from patients stung by Ca and venomous Cx collected in Central America occurred. This was also observed between sera of bathers stung by AaRS and stinging Aurelia, which appeared in Florida during the recent El Niño year. IgG was stimulated by several nematocyst proteins since many venom subfractions tested positive at high titers against convalescent sera. T-cell proliferation of mice primed with either Aurelia venom was positive against the homologous preparation with cross reactivity to the heterologous venom. Crude venoms of both Red Sea jellyfish metabolically stimulated cultured human hepatocytes more than their New World counterparts. This data shows that considerable similarities and differences exist in the venoms of these Old and New World Cassiopea and Aurelia medusae with the eastern species being more potent.

3. Gershwin L. Clonal and population variation in jellyfish symmetry. Journal of Marine Biological Association of the United Kingdom 1999: 79; 993-1000.
While it is generally assumed that jellyfish (Cnidaria Scyphozoa) are stably tetramerous, variation in symmetry (i.e. unimerous to octamerous) can be observed in most populations at a rate of approximately 2%, but sometimes as high as 10%. This type of variation has been observed among clonemates during strobilation in five taxa, namely Aurelia aurita, A. labiata, Chrysaora fuscescens, Pelagia colorata, and Phacellophora camtschatica. It is currently unclear whether the symmetry variation is caused by genetic, environmental, or developmental factors, or some combination. Although hexamerous lineages were not bred successfully, lineages were raised with rates of variation higher than normal. Thus, there may not be some genetic component to the variation. In one lineage observed over 4.5 months, the rate of non-tetramery declined substantially from an initial high of 88.9% to a final cumulative low of 29%. Apparently this lineage was able to stabilize tetramery over time, possibly indicating some self-correcting developmental mechanism. Furthermore, no difference was found in variation rates between stressed and unstressed polyps, indicating that environmental factors may not play an important role in symmetry determination in these animals. These results indicate stabilizing selection in controlling the expression of variable symmetry.

1. Masasahi Mizuno, Kazuhiro Nishikawa, Yukio Yuzawa, Tami Kanie, Hijiro Mori, Yasutetsu Araki, Nigishi Hotta, and Seiichi Matsuo, Acute renal failure after a sea anemone sting. American Journal of Kidney Diseases, 36, 2000: pE10

A 27-year old man suffering from severe swelling and pain in his right arm was examined. He showed signs of acute renal failure (ARF) with severe dermatitis of his right arm. Three days before being admitted, he accidentally touched some kind of marine organism with his right hand while snorkeling in the Sulu Sea around Cebu Island. Within a few minutes, he was experiencing severe pain in his right hand which gradually became swollen. The marine creature responsible for this injury was thought to have been a sea anemone. Histologic findings of a renal biopsy indicated that acute tubular necrosis (ATN) had caused ARF in this patient's case. Supportive therapies improved renal function of this patient, and steroid pulse therapy attenuated the severe skin discoloration. The ATN was thought to have been caused by the poison from a sea anemone. This is the first time that a marine envenomation case has been reported in which the sting of a sea anemone has caused ATN organ failure.

2.   Faisal F. Y. Radwan, Joseph W. Burnett, David A. Bloom, Tracy Coliano, Mohyee E. Eldefrawi, Holly Erdely, Laure Aurelian, Monica Torres, Edgar P. Heimer-de la Cotera. A comparison of the toxinological characteristics of two Cassiopea and Aurelia species. Toxicon 39: 2001; 245-257
A comparison of the toxinological properties of nematocyst venom from Old and New World Cassiopea and Aurelia species was undertaken. The cnidom of venomous Cassiopea andromeda (Ca) and Aurelia (AaRS) from the Red Sea was identical to that of nonvenomous Bahamian Cassiopea xamancha (Cx) and Chesapeake Bay Aurelia aurita (AaCB), respectively. A clean nematocyst preparation of Ca and both Aurelias could be obtained but algal particles could not be separated completely from the Cx nematocysts.   Further purification of all four nematocyst preparations showed significant differences in the action of the protein. Only the Cassiopea had coexisting dermonecrotic and vasopermeability producing properties and Ca's hemolytic activity was associated with mouse lethality. The protein,

Supplement 1.to ACTM Bulletin  Vol 10 No.1 - MARCH 2001

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